S0192
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S0192
Sigma
Stemline® II Hematopoietic Stem Cell Expansion Medium
Serum-free, contains L-glutamine, liquid, sterile-filtered, cell culture tested
Description
| General description | The second generation of Sigma's hematopoietic stem cell expansion media family, Stemline II has been developed to optimize the balance of differientiated and undifferentiated cells while maximizing their expansion. Compatible with Hematopoietic Stem Cells (HSC) from bone marrow, cord blood, and mobilized peripheral blood, Stemline II has been shown to lead to significant increases in cell expansion from all three sources. Through flow cytometric analysis of clinical-scale expansions, Stemline II has also demonstrated a higher capacity than other commercially available media for the expansion of CD34+/CD38+ late progenitors required for short-term engraftment. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline's utility in true functional trial. Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents. Produced in a GMP state-of-the-art facility with an available device master file (DMF), Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for clinical applications. |
| Reconstitution | Must be supplemented with growth factors and cytokines according to individual user protocols. |
| Legal Information | Stemline is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| sterility | sterile-filtered |
| form | liquid |
| quality | Drug/Device Master File available |
| suitability | cell culture tested |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | B |
| WGK Germany | 3 |
Related Products
| Related product | Z701475, Stem Cells Handbook |
References
| reference | Choong, M. et al, MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis Exp. Hematol. 35, 551-564, (2007) Abstract |
| Levay, K. et al, Tescalin is an essential factor is megakaryocytic differentiation associated with Ets family gene expression J. Clin. Invest. 117, 2672-2683, (2007) Abstract | |
| Lu, S. et al, Generation of functional humangioblasts from human embryonic stem cells Nature Methods 4, 501-509, (2007) Abstract | |
| Mc. Niece, I. et al, Delivering cellular therapies: lessons learned from ex vivo culture and clinical applications of hematopoietic cells Semin. Cell. Dev. Biol. 18, 839-845, (2007) Abstract | |
| Stec, M. et al, Expansion and differentiation of CD14++CD16+ human monocyte subsets from cord blood CD34+ hematopoietic progenitors J. Leukoc. Biol. 82, 594-602, (2007) Abstract | |
| Wulf-Goldenberg, A., et al, Cytokine pre-treatment of CD34+ cord blood stem cells in vitro reduces long-term cell engraftment in NOC/SCID mice Eur. J. Cell Biol. 87, 69-80, (2007) Abstract |
